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Pyridoxal 5′‐phosphate (PLP) biosynthesis is essential for the survival and virulence of Mycobacterium tuberculosis (Mtb). PLP functions as a cofactor for 58 putative PLP‐binding proteins encoded by the Mtb genome and could also act as a potential antioxidant. De novo biosynthesis of PLP in Mtb takes place through the ‘deoxyxylulose 5′‐phosphate (DXP)‐independent’ pathway, whereas PdxH enzymes, possessing...