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A competitive enzyme‐linked immunosorbent assay was developed to quantitate vitellogenin (VTG) in plasma and serum of coho (Oncorhynchus kisutch) and chinook (O. tshawytscha) salmon. The working range of the assay was 9 to 313 ng/ml (80–20% binding), with 50% binding at 54 ng/ml. The intra‐assay and interassay variations at approximately 50% binding were 8.1% (n = 9) and 9.0% (n = 9), respectively...