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The yeast estrogen screen (YES) assay was used to measure both estrogenic and antiestrogenic activity of wastewater treatment plant (WWTP) effluent for the purpose of developing a method to quantify antiestrogenic activity. Wastewater treatment plant effluent samples were concentrated by solid‐phase extraction (SPE) and serially diluted. Five microliters of each dilution plus 195 µl of assay medium...