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One of the main factors that can affect the quality of microarray results is the microarray hybridization specificity. The key factor that affects hybridization specificity is the design of the probes. In this paper, we described a novel oligonucleotide probe containing deoxyinosines aimed at improving DNA hybridization specificity. We compared different probes to determine the distance between deoxyinosine...
We described a novel method that combined primer extension and ligation for simultaneously detecting a set of single nucleotide variations. To verify the feasibility of this method, seven synthetic templates containing seven KRAS mutation sites and one template without mutation were detected by four primer extension cycles and one ligation reaction in the present of labeled or unlabeled nucleotides...
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