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Type-II restriction-modification (R-M) systems comprise two enzymes, a DNA methyltransferase (MTase) and a restriction endonuclease (ENase), each of which specifically interact with the same 4-8-bp sequence. All type-II MTases share several amino acid (aa) sequence motifs, which makes an evolutionary relatedness among these enzymes probable. The type-II ENases, in contrast, except for some homologous...
We have characterized a family of related restriction-modification (R-M) systems from the soil bacteriumHerpetosiphon giganteus (Hgi). A comparison of their genetic organization reveals two types of regulatory proteins, called controlling ORF C. While one of these small reading frames derived from RM HgiCI seems to be an enhancer of its own promoter, evidence is provided for a silencer function...
The polymerase chain reaction was used to produce His 6 fusion proteins via deletion of an intervening piece of DNA. The generally applicable method was performed using a standard primer with the advantage that the fusion does not produce additional amino acids. In a single-step purification highly purified, enzymatically active restriction endonuclease was obtained.
A printed version of the interactively usable genetic map of Escherichia coli K 12 is provided together with some statistical information about the actual status of the respective genome sequencing project. A total of 3179967 bp corresponding to 68,38% of the genome is available through the ECDC database. Contigs as well as individual DNA sequences for each gene or open reading frame are provided...
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