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Cyanidation wastewater contains heavy metals, including high concentrations of ammonia and free cyanide (CN‐). Aerobic growth of Fusarium oxysporum EKT01/02 in synthetic gold mine wastewater under different substrates was examined using biological stoichiometry and thermodynamic models in batch systems. The molecular weight of the dry biomass obtained was 23.03 g/C‐mol, 33.14 g C‐mol−1, and 27.06...