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To explore oxidative damage induced by PBDE 209 and its mechanism we carried out this research. Mice were given intragastrically with PBDE 209 via a metal gastric-tube at different levels (0, 50, 100, 200 mg/(kg·d)) for seven days and the malondialdehyde (MDA) content, superoxide dismutase (SOD) activities in liver and liver index were mensurated. Results showed that along with elevation of PBDE 209...
In order to study the effect of butyl benzyl phthalatee (BBP) on hepatic cells of rat, hepatic cells of rat were exposed to different dose (5 μmol / L, 20 μmol / L, 80 μmol / L) of BBP in vitro for 1 hour. Thiobarbituric acid (TBA) method , KCl-SDS precipitation method and Dinitrophenylhydrazone (DNPH) colorimetric method were used to detect malondialdehyde (MDA) content, DPC coefficient changes and...
We developed a quantitative detection method that can reflect the degree of visceral cell oxidative DNA damage induced by exogenous compound exposures using 8-OHdG as a biomarker in vitro. In this study formaldehyde (FA) was used as a model exogenous compound and malondialdehyde (MDA) was used as a reference indicator. Rat liver cell suspension was applied in this in vitro exposure experiments as...
To investigate the oxidative damage induced by di-(2-ethylhexyl) phthalate (DEHP) and analyze the possible function of such effects. This study took Esisenia foelide as experimental material to detect the levels of DNA-protein crosslinks (DPC), the contents of malondialdehyde (MDA), and the activity of surperoxide dismutase (SOD). The Esisenia foelide were exposed to DEHP for 2 hours in vivo with...
To study the effect of Butylbenzyl phthalate inducing DNA damages in Rat lung cells. KCl-SDS assay and comet assay were used to reveal the amount of DNA-protein crosslinks and effect of DNA damages induced by different doses of Butylbenzyl phathalate in vitro. The results showed that Butylbenzyl phathalate could cause DNA breakage at low level (10 μmol/L, P<;0.01), while at higher level (40 μmol/L,...
Owing to its good flame-retardant effect, polybrominated diphenyl ethers (PBDEs) were widely added to electronic, electrical appliances, as well as textiles and chemical products. Decabromodiphenyl Ether (BDE-209) is one class of PBDEs which was most demanded in international market. To illustrate the possible function of BDE-209 on rat liver, hepatic cells were expose to BDE-209 with different concentrations...
To illustrate the possible function of genotoxicity of BBP on DNA, this study took mice livers as experimental materials to detect the levels of DNA-protein crosslinks (DPC), the activity of surperoxide dismutase (SOD), and the contents of malondialdehyde (MDA) with intraperitioneal injection of different doses of BBP for two consecutive 14 days. DPC levels in hepatic cells showed a dose-dependent...
How does inhaled formaldehyde transfer the genotoxicity from exposure organ to site distant organs via blood? This is the key question to understand leukemia induced by formaldehyde. To answer this question, we used new born calf serum (NBS) as "blood" and Hela cells as materials to investigate its effects on cellular thiol concentration and DNA-protein crosslink (DPC) induced by formaldehyde...
The objective of this study is to explore the relation between oxidative stress and DNA-protein crosslink formation caused by formaldehyde (FA) inhalation in mice liver. 15 Kun Ming mice were divided into 3 groups: control group, 3.0 mg/m3 gaseous FA inhaled group, 3.0 mg/m3 gaseous FA and lipoic acid (LA) treated group. The content of reduced glutathione (GSH) and the content of DPC were measured...
One kind of nanosize titanium dioxide (nano-TiO2) was successfully made through anodization of titanium in this study, and its cytotoxicity and OS-mediated toxicity was detected by MTT, SOD and MDA assay in vitro and in vivo. The results showed that high does (0.8, 1.6 mg/mL) of this nano-TiO2 can cause cytotoxicity and OS-mediated toxicity, and reverse dose-rate effect happened in the low and medium...
In the past decade, rapid development happened in the field of nanotechnology. Recently, nano-material, especially inorganic hybrid molecule, has become an increasingly hot issue in medicine area for safe drug delivery system. In this paper, nano-layered double hydroxides (nano-LDHs) were chosen to be a potential drug carrier. In order to explore the biocompatibility of nano-LDHs with Hela cells,...
Graphitic nanomaterials have been synthesized by chemical vapor deposition in different morphologies, sizes, and shapes. After being administered to cancerous HeLa cell cultures for 24 h, it was found that they were up taken inside various sub-compartments of the cells. The surface charge was observed to play a major role if the cells were absorbed with predilection inside the cytoplasm or the nucleus...
To establish a method detecting the expression of vanilloid receptor type 1 (VR1) and explore the physiological role of VR1 as a possible bio-target molecule of formaldehyde (FA), VR1+-HEK293 (human embryonic kidney, HEK) cell model was constructed using the VRl-cDNA plasmid through the molecular cloning and transfection technology. And then, testing of capsaicin and FA-induced cell death rate in...
The purpose of the present study was to examine levels of mRNA for DNA methyltransferases (DNMTS) and methyl- CpG-binding proteins 2 (MeCP2 ) in the lung of mouse after middle-level gaseous formaldehyde exposure. Male mice were exposed to clean air or 3.0 mg/m3 formaldehyde for consecutive 72 h and the lungs were dissected for RNA extraction. The mRNA levels of dnmtl, tin in t3a and mecp2 gene were...
Objective: To explore the DNA damage of brain cells of rat after formaldehyde exposure. Methods: Rat brain cells were exposed to different concentrations of liquid formaldehyde (0.005, 0.025, 0.125 and 0.625 mmol/L) for lh. Single cell gel electrophoresis technique (comet assay) was used to test the DNA damage of the brain cells. Result: Formaldehyde could significantly cause DNA strands breakage...
In order to investigate the influences of formaldehyde with different doses on the cellular proliferation and cell cycle of the HEK293 cells, methyl thiazol tetrazolium (MTT) assay and flow cytometry were applied to measure the cellular proliferation and cell cycle. After the HEK293 cells were treated by low dose of formaldehyde (60mumol/L) for 12 hours, the percentage of phase Gl (28.4%) was decreased...
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