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Lipoxygenases are a family of nonheme iron‐containing dioxygenases. An Escherichia coli expression system producing the bacterial chaperones GroES and GroEL was engineered and successfully used to produce large quantities of recombinant human 12R‐LOX (LOXR; MW 80.34 kDa; 701 amino‐acid residues). The co‐overproduction of the two chaperones with 12R‐LOX resulted in increased solubility of 12R‐LOX and...