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Dihydrodipicolinate synthase (DHDPS; EC 4.2.1.52) catalyzes the rate‐limiting step in the (S)‐lysine biosynthesis pathway of bacteria and plants. Here, the cloning of the DHDPS gene from a clinical isolate of Streptococcus pneumoniae (OXC141 strain) and the strategy used to express, purify and crystallize the recombinant enzyme are described. Diffracting crystals were grown in high‐molecular‐weight...