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The strategies for important node detection according to topological structures are widely explored in complex networks. The diameter is a very important topological parameter among various network topological indicators. However, it is seldom utilized in important node searching methods. In this study, we defined the nodes on the diameter paths as central nodes and proposed a Diameter Center Detection...
The auxin response factors are key regulators for growth and development of plant. In this study, a total of 32 BrARF genes that distributed on the ten chromosomes were identified in rape genome. ARF gene family of Rape is expanded as compared to the ARF genes of rice (25) and arabidopsis (23). Sequence alignment and phylogenetic analysis showed that some BrARF proteins fall into related sister pairs,...
Somatic embryos were induced from cotyledon explants cultured on MS medium supplemented with 1.0 mg·L−1 2,4-D. Morphological and protein changes were performed aiming at the developmental process of somatic embryogenesis of E. sativa. The observation revealed that the embryos pass through the characteristic globular, heart, torpedo, and cotyledon stages. The developmental process of the somatic embryo...
Modification of histone proteins is associated with chromatin accessibility which profound epigenetic effect on DNA transcription, repair and replication. The SET domain proteins are recognized as regulating gene expression through lysine residues of histione methylation. Previous reports classified the SET domain proteins for 5 classes and 19 groups. In this study, we identified 15 barley SET domain...
Objective: To investigate the effect of apolipoprotein E gene polymorphism and flight hour on blood lipids level of aircrew. Method: ApoE genotypes were determined by PCR-RFLP. Plasma total cholesterol(TC), HDL cholesterol, triglycerides(TG) and LDL cholesterol were measured by standard enzymatic kits. Result: Total dyslipidemia prevalence of male aircrew (49.2%) was higher than general Chinese male...
LEF-1, lymphocyte enhancer-binding factor-1, is a member of the LEF-1/TCF (T cell-specific transcription factor) family of high mobility group (HMG) transcription factors. LEF-1/TCF is downstream of the Wnt/β-catenin signaling pathway. LEF-1 requires Wnt signaling and stabilized β-catenin to express the hair-specific keratin genes and control hair differentiation. Tcf3/LEF-1 complex has a key role...
AIM: To study the expression of survivin, an inhibitor of apoptosis protein, in human gastric carcinomas and gastric carcinoma models of rats. METHODS: With the method of immunohisto-chemical staining, we studied the expression of survivin in 20 cases of chronic gastritis and 56 cases of gastric carcinomas. We used N-methyl-N'nitro-N-nitrosoguanidine (MNNG) and high dose sodium-chloride diet to induce...
Protein-protein docking is the computational approach to predict protein-protein interactions. The 3D geometric matching plays an important role in docking because a good matching between proteins provides a good cue for searching the interactional interfaces between them. In this paper, we propose a graph-based approach to search for the protein binding site. Given two proteins, we first identify...
Biodegradable polymer polycaprolactone (PCL; Mw~100,000) fiber mats containing shikonin from the plant Lithospermumerythrorhizon, widely known for its wound healing, anti-tumor, antioxidant, and anti-inflammatory properties, were prepared via electrospinning method. Incorporation of shikonin in the neat PCL solution did not affect the morphology of the resulting fibers, as both the neat and the shikonin-loaded...
This study demonstrates that Aminoethylisothiourea (AET), a potent inhibitor of inducible nitric oxide synthase (NOS), is an irreversible competitive inhibitor of the diphenolase activity of mushroom tyrosinase when L-DOPA was assayed spectrophotometrically in vitro. The inhibition of diphenolase activity of tyrosinase by AET showed positive kinetic cooperativity which arose from the protection of...
Papain (EC 3.4.22.2) has been chemically modified using two reagents including different anhydrides of maleic and poly-maleic acids. The average ratio of modified-NH2 was tested by trinitrobenzenesulfonic acid (TNBS) method. The native and modified papain were purified by dialysis and their structures were characterized by FTIR. The factor related with the activity of the modified papain, such as...
Protein structure prediction by using bioinformatics can involve sequence similarity searches, multiple sequence alignments, identification and characterization of domains, secondary structure prediction. The secondary and tertiary structure of stearoyl ACP desaturase was predicted by bioinformatics methods from the mRNA sequence. The stearoyl ACP protein, as a member of plant fatty acid synthesis...
Amino acids (AAs) pairs in protein sheets structures have turned out to be important in studying protein tertiary structures and protein folding pathways. Most existing software tools are sophisticated in visualizing and manipulating protein 3-D structures. However, these tools are inconvenient to retrieve strands alignment information and are not so intuitive to illustrate the AAs pairs on strands...
Nylon membranes are first hydrolyed with dilute HCl and then treated with chitosan before being used as the affinity carrier. Papain as a ligand has been immobilized on the activated membranes with glutaraldehyde as a crosslinking agent. The factors involving with the activity of immobilized papain, such as concentration of glutaraldehyde, pH, temperature, reaction time, and the amount of added papain...
With the increase of sequence information, many methods have been used in detecting sequence similarities. In order to avoid the shortcomings of the traditional method based on sequences alignment such as high time and space complexity, component vector method has been successfully used in the reconstruction of phylogenetic tree using genome (proteome) or conserved molecular sequences. With the aim...
In this paper, a kind of Love wave sensors with SiO2/36degYX LiTaO3 structures for monitoring antibody-antigen immunoreactions in aqueous solutions in real time is presented. In this study, the devices with low insertion losses are achieved when the devices are in direct contact with the solutions. In the immunosensing measurements, a goat anti human-immunoglobulin G (HigG) is immobilized on the device...
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