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Polyvinylpyrrolidone (PVP) has been utilized in intracytoplasmic sperm injection (ICSI) for immobilization and manipulation of spermatozoa. This study aims to determine the suitable time that sperm cells could be safely exposed to PVP during ICSI procedure. Twenty‐five normal semen samples were prepared using the swim‐up method and then were exposed to 10% PVP at different time intervals (15, 30 and...