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While electroporation has been widely used as a physical method for gene transfection in vitro and in vivo, its application in gene therapy of cardiovascular cells remains challenging. Due to the high concentration of ion‐transport proteins in the sarcolemma, conventional electroporation of primary cardiomyocytes tends to cause ion‐channel activation and abnormal ion flux, resulting in low transfection...
On page 5971, D. Gallego‐Perez, W. Lu, L. J. Lee, and co‐workers report an advanced Bio‐NEMS (nanoelectromechanical system) device for high‐throughput cell trapping and gene delivery on a silicon‐nanochannel array chip. With a thin‐film hydrodynamic approach, individual cells are precisely positioned on a nanochannel array where the molecules of well‐controlled dose are delivered into each cells....
A process for functionalization of silicon for biosensing applications has been developed and has been verified by DNA attachment and hybridization. The functional monolayer is self-assembled on a hydrogen-terminated Si surface intended to be used on Si-based field-effect biosensors that are free of current drift and degradation. All defined stages of the functionalization process have been characterized...
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