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We prepared silica nanospheres 360 nm in diameter surface-modified with p-tert-butylthiacalix[4]arenes containing amine, carboxyl, and guanidinium groups. We found that these silica nanoparticles selectively adsorb model oligonucleotides and proteins. The particles modified with the macrocycle containing guanidinium fragments selectively adsorbed long-chain oligonucleotides and those modified with...