Search results for: A. A. Gulevich

Items from 1 to 15 out of 15 results

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Application of a System for Targeting Fe-Dependent Superoxide Dismutase and Choline Oxidase Enzymes to Chloroplast as a Strategy for Effective Plant Resistance to Abiotic Stresses

A. A. Gulevich, L. V. Kurenina, E. N. Baranova

Russian Agricultural Sciences > 2018 > 44 > 2 > 118-123

The plastids involved in photosynthesis and other essential metabolic processes are the cellular compartments most susceptible to abiotic stress factors with adverse effects in plants. In order to protect the chloroplast compartment, the plants of the Belyi Naliv tomato cultivar and the Darenka potato cultivar were transformed with genes for Fe-dependent superoxide dismutase and choline oxidase with...

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Ultrastructural organization of the domains in the cell nucleus of dicotyledonous and monocotyledonous plants under abiotic stress

E. N. Baranova, I. A. Chaban, N. V. Kononenko, M. R. Khaliluev, more

Russian Agricultural Sciences > 2017 > 43 > 3 > 199-206

The transformation of the structural organization of interphase nuclei of a plant cell, depending on the type of tissue, ploidy, and the action of abiotic factors of the medium, has been studied by light and transmission electron microscopy. It is shown that the location and the quantitative relationship between condensed and decondensed chromatin, the presence and localization of nuclear bodies in...

article

Obtaining of transgenic black medic plants with the pea defensin gene

M. N. Agafodorova, I. A. Klimenko, L. F. Solozhenzeva, E. N. Baranova, more

Russian Agricultural Sciences > 2017 > 43 > 1 > 11-14

To assess the potential use of pea defensin gene for protection from phytopathogens, some independent transgenic lines Medicago lupulina L. cv. Mir were obtained by agrobacterial transformation. Regenerants and their seed progeny were analyzed by PCR. To evaluate the potential resistance of the seed progeny selected by field experience (T1, which confirmed the presence of the target transgene by PCR),...

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Formation of the hypersensitivity response due to the expression of FeSOD1 gene in tomato when it is inoculated with Phytophthora infestans

E. N. Baranova, L. V. Kurenina, A. N. Smirnov, O. O. Beloshapkina, more

Russian Agricultural Sciences > 2017 > 43 > 1 > 15-21

The activity of antioxidant enzymes and ultrastructural changes in tissues inoculated with P. infestans isolate have been studied in the previously developed independent transgenic lines of tomato with FeSOD1 gene and control plants. It is shown that the activity of superoxide dismutase is significantly higher in transgenic plants than that in control plants (nontransgenic plants). Chlorosis and obvious...

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Analysis of obtained chloroplast transformants of tobacco (Nicotiana tabacum L.) plants with marker expression cassette of aadA ^au gene, changing the color of leaves

S. A. Danilova, G. N. Raldugina, Ye. A. Kunakova, A. A. Gulevich, more

Russian Agricultural Sciences > 2014 > 40 > 6 > 411-416

Transplastomic tobacco Nicotiana tabacum cv. Petit Havana plants were obtained by ballistic transformation. The modified expression cassette aadA ^au was used as a marker, which confers the resistance to streptomycin and spectinomycin and causes the golden color of the leaf when planted in the soil after selection on selective medium. Plants were transferred into soil and screened for the golden...

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Ultrastructural organization of chloroplasts and mitochondria of transgenic tomato plants expressing the FeSOD1 gene from Arabidopsis thaliana (L.) Heynh. under salt stress

E. N. Baranova, E. K. Nodel’man, L. V. Kurenina, A. A. Gulevich, more

Russian Agricultural Sciences > 2014 > 40 > 6 > 426-431

Ultrastructural organization of chloroplasts and mitochondria of transgenic tomato plants expressing the FeSOD1 gene from Arabidopsis thaliana (L.) Heynh. with the pea rbcS gene leader sequence under chloride and sulfate salinity has been studied. Dark respiration of transgenic tomato regenerants under stress conditions has been estimated. It has been established that expression of the FeSOD1 gene...

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Dark respiration rate of transgenic tomato plants expressing FeSOD1 gene under chloride and sulfate salinity

Ye. N. Baranova, E. N. Akanov, A. A. Gulevich, L. V. Kurenina, more

Russian Agricultural Sciences > 2014 > 40 > 1 > 14-17

Dark respiration of transgenic tomato regenerants expressing Fe-dependent superoxide dismutase under conditions of sulfate and chloride salinity has been studied. The method and system of measuring the dark respiration rate by means an infrared gas analyzer have been modified, making possible the use of individual regenerants in vitro in a small volume test tube. It is shown that transgenic lines...

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The influence of aluminium on the structural organization of the roots of barley in culture in Vitro

E. N. Baranova, A. V. Bakulina, I. G. Shirokikh, O. N. Shuplechova, more

Russian Agricultural Sciences > 2011 > 37 > 5 > 358-363

The structural organization of roots, induced in the callus cultures of barley genotypes differing in aluminum tolerance: cv Kupetz, cv 999-93 and its somaclonal form R 999-93 was studied. The goal of this research was to determine how the toxic effects of aluminum at low pH affects the structure of the root and any cells damage of different tissues meristematic zone. At pH 5.8–6.0 root callus from...

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Effects of NaCl, Na₂SO₄ and mannitol on utilization of storage protein and transformation of vacuoles in the cotyledons and seedling roots of alfalfa (Medicago sativa L.)

E. N. Baranova, A. A. Gulevich, E. B. Kalinina-Turner, N. N. Koslov

Russian Agricultural Sciences > 2011 > 37 > 1 > 11-19

The effects of NaCl, Na₂SO₄ and mannitol on utilization of storage protein in the cells of cotyledon mesophyll and root meristem during the seed germination of alfalfa (Medicago sativa L.) on the solutions of these substances at different concentrations and identical osmotic pressure were investigated using the transmission electron microscopy technique. Ultrastructural analysis revealed changes in...

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Activity of the photosynthetic apparatus and antioxidant enzymes in leaves of transgenic Solanum lycopersicum and Nicotiana tabacum plants, with FeSOD1 gene

E. N. Baranova, E. K. Serenko, T. I. Balachnina, A. A. Kosobruhov, more

Russian Agricultural Sciences > 2010 > 36 > 4 > 242-249

Introduction of the FeSOD gene enhanced the stability of the photosynthetic apparatus of plants to the action of oxidative stress caused by UV irradiation. The expression of Arabidopsis thaliana FeSOD gene, targeting the enzyme in chloroplasts due to a signal sequence, leaded to significant changes in ultrastructure of cell subcompartments of tobacco and tomato leaves. The activity of superoxide dismutase...

article

Ultrastructural evaluation of possible results of cell selection of Medicago sativa for NaCl

A. A. Gulevich, E. N. Baranova, V. Yu. Polyakov, P. N. Kharchenko

Russian Agricultural Sciences > 2010 > 36 > 2 > 100-104

Substantial differences are revealed by the transmission electron microscopy method in the ultrastructure of cell compartments containing storage reserves in plants obtained by cell selection and control plants germinated against the background of the action of NaCl and an osmotic.

article

Structural organization of nuclei and nucleoli of wheat shoot and root meristem during germination under alkaline pH conditions

E. N. Baranova, A. A. Gulevich

Russian Agricultural Sciences > 2009 > 35 > 1 > 11-14

The state of the nuclear compartment of winter wheat shoot and root meristem during germination under high pH value conditions is analyzed. An increase in the number of amoeboid nucleoli having protuberances, disturbance of chromatin compaction, and occurrence of nuclear bodies of unknown etiology in apical meristem are noted.

article

Production of transgenic tomato plants with the Fe-dependent superoxide dismutase gene

E. K. Serenko, V. N. Ovchinnikova, L. V. Kurenina, E. N. Baranova, more

Russian Agricultural Sciences > 2009 > 35 > 4 > 223-226

Agrobacterial transformation of tomatoes by an expression vector containing the Fe-SOD gene is carried out. The transgenic status of the transformants obtained is confirmed by PCR analysis. Differences are noted in the cell cycle and ultrastructure of transgenic and nontransgenic plants under the effect of salt stress.

article

Creation of a genetic engineering design with the choline oxidase gene for improved expression in plant

A. A. Gulevich, E. N. Baranova

Russian Agricultural Sciences > 2008 > 34 > 3 > 145-148

Class-IIS restriction endonucleases were used for precise joining of DNA fragments encoding part of the sequence of the choline oxidase gene, signal sequence, and translation enhancer for creating constructs.

article

Comparative analysis of anatomic structure of cotyledons for determining salt and osmotic tolerance of modified plants

E. N. Baranova, A. A. Gulevich, V. Yu. Polyakov, P. N. Kharchenko

Russian Agricultural Sciences > 2007 > 33 > 4 > 236-238

The anatomic structure of cotyledons of clone 124 and cultivar Nadezhda during germination in various isosmotic sodium chloride and mannitol concentrations is compared. The possibility of using a quantitative method for revealing the character of salt tolerance in plants obtained by biotechnological methods is shown.

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Keywords

TRANSGENIC PLANTS (5)
SOLANUM LYCOPERSICUM (3)
FE-DEPENDENT SUPEROXIDE DISMUTASE (3)
NICOTIANA TABACUM (2)
CELL SELECTION (2)
GERMINATION (2)
SALT STRESS (2)
ULTRASTRUCTURE (2)
MEDICAGO SATIVA (1)
AADA AU (1)
FESOD1 (1)
HORDEUM VULGARE (1)
PKMS8 (1)
ABIOTIC STRESS (1)
ABIOTIC STRESSES (1)
AGROBACTERIAL TRANSFORMATION (1)
AGROBACTERIUM TRANSFORMATION (1)
AL-TOLERANCE (1)
ALFALFA (1)
ANTIOXIDANT ENZYMES (1)
AUREA PHENOTYPE (1)
BALLISTIC TRANSFORMATION (1)
CHLOROPLAST ULTRASTRUCTURE (1)
CHOLINE OXIDASE (1)
CODA (1)
CONDENSATION AND DECONDENSATION OF CHROMATIN (1)
DARK RESPIRATION RATE (1)
DEFENSIN (1)
EUCHROMATIN (1)
FESOD1 (1)
FUSARIUM (1)
FUSARIUM RESISTANCE (1)
HETEROCHROMATIN (1)
HYPERSENSITIVITY (1)
INTERPHASE NUCLEUS (1)
MEDICAGO LUPULINA L. (1)
NONSPECIFIC INCLUSIONS (1)
NUCLEAR BODIES (1)
NUCLEOLUS (1)
OSMOTIC STRESS (1)
OXIDATIVE STRESS (1)
PHOTOSYNTHESIS (1)
PHYTOPHTHORA INFESTANS (1)
PHYTOPHTHORA INFESTANS CUM L. (1)
PLANT CELL (1)
PLOIDY (1)
ROOT CELL STRUCTURE (1)
SALINITY (1)
SALINITY STRESS (1)
SOLANUM LYCOPERSICUM L. (1)
SOLANUM LYCOPERSIPERSENSITIVE (1)
STORAGE PROTEIN UTILIZATION (1)
STORAGE VACUOLE (1)
SUPEROXIDE DISMUTASE (1)
SUPEROXIDE DISMUTASE FE-SOD (1)
TOMATO (1)
TOMATOES (1)
TRANSPLASTOMIC PLANTS (1)
ULTRA-STRUCTURE (1)
ULTRASTRUCTURAL ORGANIZATION OF CHLOROPLASTS AND MITOCHONDRIA (1)
ULTRASTRUCTURE OF CHLOROPLASTS (1)
UTILIZATION OF STORAGE RESERVES (1)
VARIABLE FLUORESCENCE (1)
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Search results for: A. A. Gulevich

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