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Gene expression studies have become valuable tools to identify the complex mode of action of toxic or carcinogenic substances. In our group we established a quantitative high-throughput RT-qPCR technique using Fluidigm dynamic arrays on the BioMark™ System to analyze the expression level of 95 genes in 96 samples in parallel. By creating a special gene set comprising critical genes of the DNA damage...
Predictive test systems to assess the mode of action of chemical carcinogens are urgently required. Within the present study, we applied the Fluidigm dynamic array on the BioMark™ HD System for quantitative high-throughput RT-qPCR analysis of 95 genes and 96 samples in parallel, selecting genes crucial for maintaining genomic stability, including stress response as well as DNA repair, cell cycle control,...
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