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To obtain fbpB–esxA fusing gene of Mycobacterium tuberculosis (MTB), express the encoded fusing protein in Escherichia coli (E. coli), identify protein acquired, and predict the structure and function of the protein utilizing methods of bioinformatics. fbpB and esxA gene were amplified from genome of MTB H37Rv by PCR. The fbpB–esxA fusing gene ligated by (Gly 4 Ser) 3 linker was...
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