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This study aims to produce and apply a biosurfactant from Gordonia westfalica GY40 for enhancing fuel oil solubilization and degradation in seawater. The immobilization of G. westfalica GY40 cells on chitosan flakes increased biosurfactant yield, and we achieved a biosurfactant concentration as high as 1.85 g L−1 when using 2 % soybean oil as the carbon source. The critical micelle dilution (CMD)...