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Preparation of nanocrystalline cellulose (NCC) by 62 and 65 % wt. sulfuric acid hydrolysis of cellulase-pretreated fibers was optimized to obtain the highest yield by applying two statistical plans. At optimal conditions (10 U/g odp cellulase, 25 min hydrolysis, 47 °C, 62 wt.% H2SO4), high yields (≥80 %) were obtained, including an increase of ~9 points due to the enzyme. Optimal conditions produced...
In this work, treatments with a xylanase (X) and carbohydrases mixture (Cx) were applied on a TCF bleached sisal pulp in order to obtain high-cellulose content fibers applicable on a wide range of uses. A limit of ≈12% w/w final content in hemicelluloses was found regardless of the enzymatic treatment assessed. An extraction with 4% and 9% w/v NaOH was performed for further hemicelluloses removal...
In this work the introduction of a cellulase treatment prior to NCC isolation was assessed. NCC was produced using sulfuric acid at two different concentrations (62 and 64% wt.). The effect of pore size for filtration step was also assessed. The smaller acid dose leaded to yields up to 65–70% and average size up to 160nm. It also produced crystals with reduced sulfur content (0.6–1%). Cellulase pretreatment...
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