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A fluorometric method is described for the detection of alkaline phosphatase (ALP) activity. It is based on the use of the product of hydrolysis of the drug amifostine (a thiophosphoester) by ALP. It is known that MnO2 nanosheets quench the blue fluorescence of tungsten disulfide quantum dots (WS2 QDs) which have excitation/emission wavelengths of 320/448 nm. However, in the presence of ALP and amifostine,...
A turn-on fluorometric method is described for selective and sensitive detection of cysteine (Cys). Gold nanoclusters (Au NCs) capped with glutathione (GSH) are used as a fluorescent probe. If Ce3+ ion are present, they will bind to the carboxy groups of the GSH-capped Au NC. This results in aggregation-induced emission enhancement (AIEE), best measured at excitation/emission wavelengths of 360/575 nm...
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