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A rapid method is described for the preparation of a highly uniform and sensitive SERS substrate by an improved ‘drop-and-dry’ method. Gold nanobipyramids (Au NBPs) were prepared inside the nanoholes (nanowalls) of anodic aluminum oxide (AAO) templates with a typically 5-μm nanohole depth. The SERS substrate can be prepared by this method within 40 s and on large scale. The SERS signals obtained with...