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We present experimental evidence and a tentative explanation for a recently observed phenomenon in 532 nm picosecond laser selective thin-film ablation. By gradually increasing the laser pulse overlap in thin metal patterning at fluences just above the single-pulse ablation threshold, the ablated feature quality degrades steadily until an overlap limit beyond which ablation, surprisingly, terminates...
Current frontier of cellular biology is the manipulation, analysis and sorting of single cells. Populations of cells in culture and in organisms, although considered nominally identical, often present some heterogeneity whose analysis may unravel the complexity of many biological phenomena [1]. Optical stretching is a powerful technique to study the mechanical properties of single suspended cells...
We present an optofluidic monolithic chip for the study of mechanical properties of single cells by means of optical forces. The chip is fabricated by femtosecond laser micromachining in a fused silica glass substrate
The investigation of the mechanical properties of individual cells is of great interest for the comprehension of basic biological mechanisms and for the early detection of several diseases. Optical stretchers based on fibers have widely demonstrated their effectiveness for this kind of analysis [1]. Nevertheless, in order to turn such a device into an operative tool for biological studies, it is essential...
We present a monolithic chip able to perform single-cell mechanical analyses without physical contact and with high reproducibility. The chip is based on a fused silica glass substrate and it is fabricated by fs-laser micromachining.
Lab-on-a-chips (LOCs) are becoming one of the most powerful tools of analytical chemistry, with a broad application in life sciences, biotechnology and drug development. They incorporate in a single substrate the functions of a biological laboratory (i.e. microfluidic channels, reservoirs, valves, pumps and sensors). Their main advantages include the possibility of working with small sample quantities...
We report on innovative application of microchannels, fabricated by femtosecond laser irradiation followed by chemical etching. This technique allows us to demonstrate a novel approach to the achievement of organic photonic devices, where the unique properties of a conjugated polymer in solution are exploited in a microfluidic configuration in order to produce an easy-to-integrate photonic device...
A novel femtosecond Yb-fiber based amplifier seeded with a cavity-dumped Yb:KYW laser oscillator is employed for the writing of waveguides. The presented results have been achieved with a pulse energy of 1.6 muJ at 420 fs and a repetition rate of 1 MHz. We created simultaneously written waveguide pairs in fused silica with separations varying from 175 mum to 6 mum, using a 20x microscope objective...
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