Search results for: Barkha Ratta

Items from 1 to 11 out of 11 results

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Bovine Herpes Virus 1 Major Immediate Early Transcription Unit 1 (IETU-1) Uses Alternative Promoters to Transcribe BICP0 and BICP4 Transcripts

Mayank Pokhriyal, O. P. Verma, Barkha Ratta, Ajay Kumar, more

Current Microbiology > 2016 > 72 > 4 > 420-425

Immediate early (IE) genes are transcribed immediately after infection in BHV1 from two different immediate early transcription units. It is reported that the immediate early transcription unit I (IE TU1) of Bovine herpesvirus 1 (BHV1) transcribes two proteins BICP0 and BICP4 from a single promoter by alternative splicing but with identical 5′UTR. We found that the transcripts of BICP0 and BICP4 have...

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Detection of Peste Des Petits Ruminants Virus (PPRV) Genome from Nasal Swabs of Dogs

Barkha Ratta, Mayank Pokhriyal, Shanker K. Singh, Ajay Kumar, more

Current Microbiology > 2016 > 73 > 1 > 99-103

Peste des petits ruminants virus (PPRV) one of the most important viruses of small ruminants has a restricted host range. We report here the presence of PPRV virus in the nasal swabs of 3 out of 12 dogs in a routine microarray screening. The presence of PPRV sequence was further confirmed by PCR and sequencing. The sequence analysis revealed that the PPRV virus has close similarities with the viruses...

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Microarray Chip Based Identification of a Mixed Infection of Bovine Herpesvirus 1 and Bovine Viral Diarrhea 2 From Indian Cattle

Barkha Ratta, Brijesh Singh Yadav, Mayank Pokhriyal, Meeta Saxena, more

Current Microbiology > 2014 > 68 > 1 > 127-131

Bovine herpesvirus 1 (BHV1) and bovine viral diarrhea virus 2 (BVD2) are endemic in India although no mixed infection with these viruses has been reported from India. We report first mixed infection of these viruses in cattle during routine screening with a microarray chip. 62 of the 69 probes of BHV1 and 42 of the 57 BVD2 probes in the chip gave positive signals for the virus. The virus infections...

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Erratum to: HN protein of Newcastle disease virus causes apoptosis in chicken embryo fibroblast cells

P. V. Ravindra, Ashok K. Tiwari, Bhaskar Sharma, Yogendra Singh Rajawat, more

Archives of Virology > 2014 > 159 > 8 > 2205-2205

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Isolation of Newcastle disease virus from a non-avian host (sheep) and its implications

Bhaskar Sharma, Mayank Pokhriyal, Gaurava K. Rai, Meeta Saxena, more

Archives of Virology > 2012 > 157 > 8 > 1565-1567

Newcastle disease virus (NDV) is an avian virus that has not been isolated from naturally infected non-avian and non-human hosts except for one report in which it was isolated from cattle in 1952. We report here for the first time the isolation and identification of NDV from sheep and suggest that this virus be included in the screening of viruses from non-avian hosts.

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Characterization and Expression of E2 Glycoprotein of Classical Swine Fever Virus in a Eukaryotic Expression System

Barkha Ratta, Binita Nautiyal, P. V. Ravindra, Uttara Chaturvedi, more

VirusDisease > 2010 > 21 > 1 > 69-75

Classical swine fever (CSF) is an economically important Office International des Epizooties (OIE) list A disease of swine characterized by high fever and multiple haemmorhages. The E2 glycoprotein of CSFV is immunogenic and induces neutralizing antibodies against CSFV. In the present study, complete coding region of the E2 gene from Indian virulent field isolate (Mathura) was amplified by reverse...

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Time course of Newcastle disease virus-induced apoptotic pathways

P.V. Ravindra, Ashok K. Tiwari, Barkha Ratta, Manish V. Bais, more

Virus Research > 2009 > 144 > 1-2 > 350-354

Newcastle disease virus (NDV) causes economically significant Newcastle disease (ND) in almost all birds worldwide. Previous studies have shown that NDV induces caspase dependent apoptotic pathways in infected cells. In the present study, time course induction of apoptotic pathways in Vero cells is described. In NDV-infected cells, caspase-8 activity, percentage of cells showing TRAIL expression was...

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Newcastle disease virus-induced cytopathic effect in infected cells is caused by apoptosis

P.V. Ravindra, Ashok K. Tiwari, Barkha Ratta, Uttara Chaturvedi, more

Virus Research > 2009 > 141 > 1 > 13-20

The velogenic Newcastle disease virus (NDV) causes highly infectious and economically significant Newcastle disease (ND) in birds of various species. In cell culture NDV induces cytopathic effect (CPE) characterized by rounding, vacuolation, syncytia formation and cell death. Aside from cell to cell fusion caused by the F and HN glycoprotein of the virus molecular events leading to cell death are...

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Detection of canine adenoviral infections in urine and faeces by the polymerase chain reaction

Uttara Chaturvedi, Ashok K. Tiwari, Barkha Ratta, P.V. Ravindra, more

Journal of Virological Methods > 2008 > 149 > 2 > 260-263

The present study was aimed at developing the polymerase chain reaction (PCR) assay for detection of canine adenoviruses from faecal or urine samples. Urine or faecal samples were treated with chloroform or activated charcoal to eliminate the PCR inhibitory substances and the total DNA was extracted. The PCR was optimized using common set of primers to amplify 508bp or 1030bp DNA sequence within E3...

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Induction of apoptosis in Vero cells by Newcastle disease virus requires viral replication, de-novo protein synthesis and caspase activation

P.V. Ravindra, Ashok K. Tiwari, Barkha Ratta, Uttara Chaturvedi, more

Virus Research > 2008 > 133 > 2 > 285-290

Newcastle disease virus causes (NDV) apoptotic death of infected cells. In the present study, the stimulus that provoked the induction of apoptosis in infected cells was examined. Vero cells infected with NDV developed apoptosis as characterized by DNA fragmentation and decreased DNA content. In presence of ammonium chloride, infected cells did not show reduced DNA content indicating the requirement...

article

HN protein of Newcastle disease virus causes apoptosis in chicken embryo fibroblast cells

P. V. Ravindra, Ashok K. Tiwari, Bhaskar Sharma, Yogendra Singh Rajawat, more

Archives of Virology > 2008 > 153 > 4 > 749-754

Newcastle disease virus (NDV), an avian paramyxovirus, induces apoptosis in chicken embryo fibroblast (CEF) cells. In the present investigation, the ability of haemagglutinin-neuraminidase (HN) protein of NDV to cause apoptosis in CEF cells was examined. The results revealed that cells expressing the HN protein demonstrated decreased DNA content, phosphatidylserine exposure and increased cytoplasmic...

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Keywords

NEWCASTLE DISEASE VIRUS (3)
APOPTOSIS (2)
APOPTOTIC PATHWAYS (1)
CANINE ADENOVIRUS (1)
CASPASES (1)
CLASSICAL SWINE FEVER (CSF) (1)
CLASSICAL SWINE FEVER VIRUS (CSFV) (1)
CYTOPATHIC EFFECT (1)
FAECES (1)
PCR (1)
REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION (RT-PCR) (1)
TIME COURSE INDUCTION (1)
URINE (1)
VERO CELLS (1)
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Elsevier (4)

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Search results for: Barkha Ratta

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