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The bacterial expression of human progastrin6–80 has been reported previously [Baldwin, G.S. et al. (2001) J. Biol. Chem.276: 7791-7796]. The aims of the present study were to prepare full-length recombinant human progastrin1–80 and to compare its biological activity with that of progastrin6–80in vitro, to determine whether or not the N-terminal five amino acids contributed to activity. A fusion...