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Obtaining high-quality crystals for X-ray diffraction from membrane proteins has proven to be a difficult task. One recently presented method utilises the cubic phases formed by 1-monooleoyl-rac-glycerol (MO). Removing the proteins from their native environment requires the use of surfactants. One commonly used surfactant is n-octyl-β-D-glucopyranoside (OG). Using NMR techniques and visual observations,...
Obtaining high-quality crystals for X-ray diffraction from membrane proteins has proven to be a difficult task. One recently presented method utilises the cubic phases formed by 1-monooleoyl-rac-glycerol (MO). Removing the proteins from their native environment requires the use of surfactants. One commonly used surfactant is n-octyl-β-D-glucopyranoside (OG). Using NMR techniques and visual observations,...
Competition between mono- and divalent ions in the association of counterions to the headgroups of amphiphiles was studied in one surfactant system with organic counterions (piperidine + /piperazine 2+ octanesulfonate) and one with inorganic counterions (Na + /Ca 2+ octyl sulfate). By conductivity and 13 C NMR chemical shift measurements the critical micelle...
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