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Abstract The gene loci ech, encoding enoyl-CoA hydratase/aldolase, and fcs, encoding an unusual feruloyl-CoA synthetase, which are involved in the bioconversion of ferulic acid to vanillin by the Gram-positive bacterium Amycolatopsis sp. strain HR167, were localized on a 4,000bp PstI fragment (P40). The nucleotide sequence of P40 was determined, revealing open reading frames of 864bp and 1,476bp,...