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Chrysanthemum (Chrysanthemum morifolium Ramat.) is one of the most popular ornamental flowers in the world, and many agronomic traits have recently been introduced to chrysanthemum cultivars by gene transformation. Concerns have been raised, however, regarding transgene flow from transgenic plants to wild plants. In early studies, ethylene receptor genes have been used for genetic modification in...
We produced intergeneric somatic hybrids between chrysanthemum [Dendranthemaxgrandiflorum (Ramat.) Kitamura] and wormwood (Artemisia sieversiana J. F. Ehrh. ex. Willd) by electrofusion of mesophyll protoplasts. Chrysanthemum protoplasts were inactivated with iodoacetamide for 15min at 5 o C and electrofused with wormwood protoplasts. The fused cells were cultured on MS basal medium containing...
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