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. We have studied the properties of a non-selective cation current (NSCCa) in macrovascular endothelial cells derived from human umbilical vein (EA cells) that is activated by an increase of intracellular Ca2+ concentration, [Ca2+]i. Current–voltage relationships are linear and the kinetics of the current is time-independent. Current–[Ca2+]i relationships were fitted to a Ca2+ binding site model...