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Key messagePhase change-related epigenetic and physiological changes in the micropropagation process ofBetula platyphylla. Abstract To evaluate the epigenetic regulation and the cell redox state during micropropagation, Betula platyphylla was used to regenerate plants from callus. Axillary bud, callus (20 days after induction), aged callus (40 days after induction), budding callus, regenerating...
For cross-pollination trees, the optimal breeding method is hybridization. Tree heterosis is commonly present and is the main research focus in tree crossbreeding. Salt stress and interspecific hybridization may lead to DNA methylation changes. The study crossed Fraxinus mandshurica (female parent) with Fraxinus velutina (male parent) to obtain interspecific F1 hybrid progenies that could obtain the...
Key MessageThe advantages of F1 hybrid progenies ofFraxinus mandshuricaandFraxinus americanawere analyzed under drought conditions in the field and greenhouse. Abstract For cross-pollination trees, the optimal breeding method is hybridization. Tree heterosis is commonly present and is the main research focus in tree crossbreeding. In this study, Fraxinus mandshurica was used as female...
The water quality in rainfall, throughfall, stemflow and litterthrough in the Fraxinus mandshurica at Eastern Mountainous Region in Heilongjiang Province had been studied. The indicator included pH, Do, TURB, TDS, COND, ORP, K, Na, Ca, Mg, Cu, Zn, Mn and Fe. The results showed that the chemical properties of rainfall has been changed after it passing through forest canopy. The value of Do and ORP...
Studies about the variation of DNA methylation pattern under high temperature stress (37°C) contribute to a comprehensive understanding of the apparent regulation of biological functions. Based on the in vitro cultivation of genetically modified birch (Betula platyphylla Suk.) clones for high-temperature stress, the paper detected exogenous gene transcription level differences with RT-PCR. Methylation-sensitive...
The integration and structure of a transgene locus can have profound effects on the level and stability of transgene expression. We screened 28 transgenic birch (Betula platyphylla Suk.) lines transformed with an insect-resistance gene (bgt) using Agrobacterium tumefaciens. Among the transgenic plants, the copy number of transgene varied from one to four. A rearrangement or partial deletion had occurred...
In this paper, Southern blot analysis of the transgenic birch (Betula platyphylla Suk.) plants indicated that the the copy number of bgt varies from one to four. Of which 18.2 percent were single copy. Transcriptional expression of bgt gene in transgenic birch plants was analyzed by Northern blot. TGS has been found in four transgenic lines with one, three and four copies of bgt. Bgt gene was inserted...
Long-lived tree mediated by Agrobacterium tumefaciens has technically matured to some extent, but the mechanics of T-DNA integration in transgenic birch (Betula platyphylla) remains largely unknown. Using SiteFinding PCR, we analyzed the flanking sequences of T-DNAs in transgenic birch plants, in which the foreign genes have been integrated stably. Flanking sequence analysis was conducted for the...
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