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The first genome of a virus infecting a representative of the eubacterial genus Actinoplanes is presented. Phage φAsp2 has a circularly permutated chromosome that consists of 58,638 bp; its G/C-bias of 70.39% resembles the hosts G + C-content (71–73% within the genus). A total of 76 open reading frames (orfs) were identified, the majority of which (63) displaying equal transcriptional orientations...
Crude extracts of Actinoplanes missouriensis and related strains catalyze the ATP-dependent phosphorylation of maltose to maltose 1-phosphate. The enzyme of A. missouriensis responsible for this reaction was purified and characterized. This protein has an estimated molecular mass of 57kDa and it is most likely a monomer. The Km value was 2.6mM for maltose and 0.54mM for ATP. Only maltose acted effectively...
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