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Abstract A sugarcane juice-based low cost culture medium was previously explored to produce the carotenoid pigment astaxanthin in liquid culture by the red yeast Phaffia rhodozyma (1300g astaxanthin/g of dry yeast and 6500g/l whole culture medium). Two peculiar limitations in Phaffia are growth temperature (26C) and lack of sugar osmotolerance. Two advantages are the wide biochemical ability for the...