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Taguchi’s L8 experimental design was firstly applied to identify critical parameters affecting the efficiency of bridge formation between confluent and fresh microcarriers in bead-to-bead transfer of Vero cells. Statistical analysis indicated that stirring time, rest time and the interactions between stirring time and rest time, stirring speed and rest time had significant effects. Subsequently, a...
A 2-level full factorial design was firstly employed to explore the responses of murine bone marrow mesenchymal stem cells stimulated by various combinations of EGF, PDGF-BB, and fibronectin. EGF and PDGF-BB individually, rather than fibronectin, had significant effects on cell proliferation. The synergism between PDGF-BB and fibronectin, as well as the antagonism between EGF and PDGF-BB were also...
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