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Many-year data (1992–2010) of bioassay studies were used to identify the dynamics of surface water toxicity in water bodies of the Lower Don in Rostov-on-Don City. Segments of the Lower Don, the Temernik R. mouth, and Lake Goluboe were studied taking into account the anthropogenic load typical of them. The toxicity was evaluated by bioassay tests with four test-objects with different trophic levels...