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Thermoprecipitation of lysozyme from egg white was demonstrated using copolymers of N-isopropylacrylamide with acrylic acid, methacrylic acid, 2-acryloylamido-2-methylpropane-sulfonic acid and itaconic acid, respectively. Polymers synthesized using molar feed ratio of N-isopropylacrylamide:acidic monomers of 98:2 exhibited lower critical solution temperatures in the range of 33-35 o C. These...
N-Isopropylacrylamide (NIPAM) and O-vinyladipoyl uridine were copolymerized in different feed molar ratios ca. 95:5 to 75:25, respectively. The resulting polymers were characterized by gel-permeation chromactography, differential scanning calorimeter and cloud point measurements. The number-average molecular weights of the copolymers were 1.6×104≈3.3×104. The lower critical solution temperature of...
A series of copolymers of N-isopropylacrylamide (NIPA) and acryloyl amino acids were synthesized and conjugated with p-aminobenzamidine (PABA). The values of lower critical solution temperatures (LCSTs) of these polymers estimated by 1 H NMR were in agreement with those estimated by the turbidometric methods. The apparent shifts in the peak positions for the polymer with increasing temperature...
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