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The present work shows the optimization and validation results of an analytical methodology based on imunoaffinity clean-up (IAC) followed by liquid chromatography with tandem mass detection (LC-MS-MS) for the analysis of the mycotoxins fumonisin B1 (FB1) and B2 (FB2) in human urine samples, in order to evaluate fumonisins exposure in two Portuguese populations. As far as we know, imunoaffinity clean-up...