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For elucidating protein–protein interactions, many methodologies have been developed during the past two decades. For investigation of interactions inside cells under physiological conditions, yeast is an attractive organism with which to quickly screen for hopeful candidates using versatile genetic technologies, and various types of approaches are now available. Among them, a variety of unique systems...
We have developed a new approach based on the Gγ recruitment system to screen affinity‐enhanced proteins by expressing a binding competitor. The previously established Gγ recruitment system is a yeast two‐hybrid (Y2H) system that utilizes G‐protein signaling, and is based on the fact that membrane localization of the G‐protein γ subunit (Gγ) is essential for signal transduction in yeast. In the original...
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