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We developed 12 highly polymorphic microsatellite markers for the eastern box turtle (Terrapene carolina carolina). The number of alleles per locus ranged from 5 to 27 (mean: 15.2 per locus) and the observed heterozygosities ranged from 0.371 to 0.943 among 35 individuals. Two loci exhibited significant deviations from Hardy–Weinberg equilibrium. Five loci exhibited putative null alleles but this...