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Hintergrund Die Fortschritte in der DNA-Sequenzierung ermöglichen die genetischen Auslöser von Erkrankungen zu identifizieren. Zudem ist es heute möglich, mit „CRISPR-Cas9“ („clustered regularly-interspaced short palindromic repeats und crispr-associated protein 9“) genomische DNA-Sequenzen präzise und mit hoher Effizienz zu editieren. In kürzlich veröffentlichten präklinischen Studien wurde diese...
The aim of this study has been to characterize adult human somatic periodontium-derived stem cells (PDSCS) isolated from human periodontium and to follow their differentiation after cell culture.PDSCS were isolated from human periodontal tissue and cultured as spheres in serum-free medium. After 10 days the primary spheres were dissociated and the secondary spheres sub-cultured for another 1–2 weeks...
AbstractNF-B is found in many neuronal cell types in different states of activity. This study aimed to define which conditions induce constitutive NF-B activity in cultured hippocampal neurons using activity-specific antibody staining. In co-culture with astroglia, hippocampal neurons were devoid of activated NF-B. In these co-cultures, NF-B could not be activated via kainate or glutamate. In contrast,...
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