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This study investigates the pH-dependent modulations in haemoglobin response to linoleic acid, employing a spectroscopic analysis across HbAA, HbAS, and HbSS variants. Concentration-dependent effects of linoleic acid at varying pH levels reveal nuanced behaviours in absorbance, peak maintenance, and spectra characteristics. At pH 7.2, HbAA and HbSS display increased absorbance with peak maintenance,...