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Nano device based DNA sequencing has the advantage of speed and cost when compared to the conventional Sanger methods. In this report, we demonstrate an AFM assisted DNA sequencing method that combines precision single molecule manipulation with a nanowire sensor to measure the transient tunneling current of a translocating DNA molecule. In experiment, a 7-µm long λDNA molecule was first identified...
On behalf of the organizing committee, we would like to welcome everybody to the 12th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2017)! IEEE-NEMS is a premier conference series established by the IEEE Nanotechnology Council to promote advanced research and development activities in MEMS, nanotechnology, and molecular systems. The first IEEE-NEMS...
One-dimensional (1D) carbon chains or carbynes consist of unique electronic properties that are highly suitable for electrical sensing of biomolecules such as DNA based on conductance change. In addition, the single atomic width of a carbyne enables the possibility of ultra-high spatial resolution in the measurement of individual DNA bases when compared to other carbon-based nanostructures such as...
Algae cells with natural advantages of sensing and moving at micro scale can be regarded as microrobots. While it is difficult to control the locomotion or harness the bioenergy of the cells. This study develops a novel algae guiding system to robotize the algae cell Chlamydomonas reinhardtii based on the phototaxis. Algae cells' swimming trajectory, speed and force were analyzed. Furthermore, the...
Carbon based nanostructures such as graphene and carbon nanotubes have received widespread attention due to their unique mechanical and electronic properties. This paper describes a quantum mechanics based study of the electronic band structures and transport properties of one-dimensional (1D) carbon chains, the thinnest nanowires available in nature. The study is based on the application of density...
This paper presents a new method of microfluidic chip fabrication using 3D design and 3D printing techniques. The printed materials are flexible and inexpensive. The fabrication procedure is time-saving and less complex. The water leakage and hydrophilic test are performed to prove the advantages over traditional methods. The flow rates of the printed pump chip have been tested under varied applied...
Graphene has been widely studied because of its high mobility, superior mechanical properties and excellent thermal and chemical stability. This paper describes a novel and flexible method to fabricate graphene interdigitated electrodes and all-carbon field effect transistor (FET). In this approach, graphene is first grown by chemical vapor deposition (CVD) and assembled onto a microelectrode chip...
A Pyrex nanochannel system with embedded transverse nanoelectrodes was designed and fabricated by MEMS based microfabrication and focused ion beam (FIB) nanolithography. The microchannels and microelectrodes in the system were patterned on a Cr/Au coated Pyrex wafer using conventional photolithography while the nanoelectrodes (100 nm wide and 50 nm thick) and the nanochannels (500 nm to 1 μm wide...
This paper presents a combined experimental and numerical simulation study of polydimethylsiloxane (PDMS) pneumatic micropumps. The micropumps consist of three pneumatic PDMS membranes, one pump chamber, and two check valves. In particular, two flow stoppers in fluidic channel corresponding to the check valves can avoid back flow, and increase the flow rate. The flow rates of the micropumps have been...
This paper presents a telemedicine system utilizing an integrated microfluidic chip for fast, automatic detection of insulin. The telemedicine system expedites the insulin assay results digitally from the assay site to medical professionals located off-site, while the off-site specialist can directly provide diagnosis through the Internet. The integrated microfluidic chip contains pneumatic micropumps,...
This paper reports a microfluidic-based telemedicine system for the determination of insulin, an important indicator for clinical diagnosis of diabetes. The telemedicine system conveys the assay results digitally to established healthcare facilities, where trained medical professionals can directly assist the detection process and provide diagnosis through the Internet. The integrated polydimethysiloxane...
Welcome to the Second Annual IEEE International Conference on Cyber Technology in Automation, Control, and Intelligent Systems (IEEE-CYBER 2012)! Cyber technology has grown tremendously in recent years as new generations of internet-friendly automation systems continue to usher in fundamental changes to our everyday lives in such areas as healthcare, energy, telecommunication, environment, and manufacturing...
A Pyrex nanochannel device was fabricated using AFM nanolithography in conjunction with MEMS-based microfabrication. The fabrication process began with the patterning of microchannels on a Pyrex substrate using photolithography and wet etching. AFM nanolithography was then performed to realize a nanochannel between the micro reservoirs. A diamond-coated AFM probe with a relatively large force constant...
This article describes a prototype microfluidic system for insulin detection and exchanging the results digitally with physicians located off-site. The integrated polydimethysiloxane (PDMS) microfluidic system uses two pneumatic micropumps and one micromixer for chemiluminescent immunoassay, and a developed communications infrastructure for transferring the digital information from the assay site...
A silicon nanochannel system with integrated transverse electrodes was designed and fabricated by combining MEMS microfabrication and AFM nanolithography. The fabrication process began with the patterning of microscale reservoirs and electrodes on an oxidized silicon chip using conventional MEMS techniques. A nanochannel, approximately 30µm long with a small semi-circular cross-sectional area of 20nm...
The objectives of this study were to improve our previous portable impedance biosensor instrument with more rapid, reliable and quantitative features for detection of avian influenza (AI) virus and to evaluate its performance using H5N1 and H5N2 virus. A high-intensity and high-gradient magnetic field based separator was designed and fabricated for rapid separation of AI virus. KPL measuring solution...
An experimental study was carried out to evaluate the performance of a polydimethylsiloxane (PDMS) based micromixer for heterogeneous immunoassays of insulin. The detection method is based on coupling the highly specific double-antibody sandwich immunoassay with the sensitive chemiluminescence of the Luminol-Hydrogen Peroxide reaction. Both the immune and chemiluminescence reactions are conducted...
The bioconjugation efficiency resulting from three PDMS based continuous flow micromixers are experimentally studied at various sample flow rates and pneumatic actuation frequencies. In this study, streptavidin-coupled paramagnetic microbeads and biotin-coupled fluorescent microbeads are separately injected into the micromixers and the resultant binding efficiency is measured by the fluorescence emission...
This paper reports the design and fabrication of a biosensor based on aligned single wall carbon nanotubes (SWCNTs) with integrated single-strand DNAs (ssDNA). This ultra-sensitive biosensor will provide label-free real-time electronic detection of DNA hybridization between surface-immobilized ssDNA and target ssDNA. Hybridization kinetics between complementary and target ssDNA nucleotide base pairs...
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