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Fluorescence microscopy enables one to visualize subcellular structures of living tissue or cells in three dimensions. This is especially true for two-photon microscopy using near-infrared light which can image deeper into tissue. To characterize and analyze biological structures, nuclei segmentation is a prerequisite step. Due to the complexity and size of the image data sets, manual segmentation...
Fluorescence microscopy has emerged as a powerful tool for studying cell biology because it enables the acquisition of 3D image volumes deeper into tissue and the imaging of complex subcellular structures. Quantitative analysis of these structures, which is needed to characterize the structure and constitution of tissue volumes, is facilitated by nuclei segmentation. However, manual segmentation is...
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