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Mass spectrometry‐based proteomics has been extensively used to map bacterial proteomes, which has led to a better understanding of the molecular mechanisms underlying bacterial infection and bacteria–host interactions. Quantitative proteomics using selected or parallel reaction monitoring is considered one of the most sensitive and specific quantitative MS‐based approaches and has significantly advanced...
Optimization of deep brain stimulation (DBS) parameters is a weeks to months-long process for many patients, due in part to the inflammatory response caused by electrode implantation. To investigate the effect of inflammation on current delivery. transgenic mice, expressing Thy1-GCaMP6f (a genetically-encoded calcium sensor), were implanted with a DBS electrode in the subthalamic nucleus (STN). Immediately...
Recent work demonstrated the efficacy of combining layer-by-layer assembly with hydrogels to provide the controlled delivery of proteins for use in nerve repair scaffolds. In this work, we augmented the protein dose response by controlling and increasing the hydrogel internal surface area. Sucrose was added to agarose during gelation to homogenize the nanopore morphology, resulting in increased surface...
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