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Providing an accurate count of total leukocytes and specific subsets (such as T-cells and B-cells) within small amounts of whole blood is a rather challenging ordeal due to the lack of techniques that enable the separation of leukocytes from a limited amount of whole blood. In a previous study we designed a microfluidic chip utilizing a micropillar array to isolate T-cells and B-cells from the sub-microliter...
Absolute counting of total leukocytes and specific subset (such as T-cells and B-cells) within small amounts of whole blood is difficult due to the lack of techniques that enables separation of leukocytes from limited volume of whole blood. In this study, a microfluidic chip equipped with a size controlled micropillar array for highly separation of T-cells and B-cells from sub-microliter of whole...
We present an automation system for on-chip cell mechanical characterization system for evaluating the stiffness of oocytes. A high frequency visual feedback control system is proposed. Using simple yet effective image analysis algorithms, the high frequency visual feedback is able to improve the response of the cell manipulation, the throughput of the system is improved consequently. To measure the...
In the study of the oocytes/embryos, such as enucleation, microinjection in order to increase the success ratio of the fertilization and characteristics study of the oocytes, all of these research and clinical applications involve 3-D rotation of mammalian oocytes. The gesture or the orientation of the oocyte is critical for improving the enucleation success rate, and characteristics investigation...
This paper presents a novel measurement system of cellular mechanical properties based on a robot integrated microfluidic chip. In order to achieve the high throughput measurement of cellular mechacal properties, we proposed the robot integrated microfluidic chip (robochip), taking advantages of both of micromechanical manipulator and Lab-on-a-Chip devices. The robochip contained a pair of a magnetically...
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