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Here we have developed a novel large-field high-resolution two-photon digital scanned light-sheet microscopy (2P3A-DSLM) based on ultrafast axial scanning of illumination focal spot with a tunable acoustic gradient (TAG) index device. This new technique provides a sub-micron axial resolution with a large field of view in deep tissues. In vivo imaging with 2P3A-DSLM resolves functional maturation of...
Many receptor-mediated endocytic processes are mediated by constitutive budding of clathrin-coated pits (CCPs) at spatially randomized sites before slowly pinching off from the plasma membrane (60–100 s). In contrast, clathrin-mediated endocytosis (CME) coupled with regulated exocytosis in excitable cells occurs at peri-exocytic sites shortly after vesicle fusion (∼10 s). The molecular mechanism underlying...
We demonstrate a new MEMS-Based Shadowless-illuminated variable-angle TIRF (SIVA-TIRF), which can realize two-plane, two-color TIRF imaging at the speed of 25Hz.
We demonstrate an 880 mW 780 nm femtosecond laser based on a high-order dispersion compensated Er-doped fiber laser and frequency doubling. It has been used on a two-photon three-axis digital scanned light-sheet microscopy.
We optimized pre-chirp in an Nd-doped fiber amplifier to achieve high-quality femtosecond 920 nm pulses. The in vivo zebrafish imaging proves the laser amplifier an ideal source in two-photon microscopic imaging.
We demonstrate a new two-photon light-sheet microscopy with three-color excitation, diffraction-limited thickness and tailorable illumination area, capable of multi-color multi-scale live imaging in one setup.
We demonstrate a new two-photon scanned light-sheet microscopy with diffraction-limited thickness and tailorable illumination area from 50×50 µm2 to 500×500 µm2, capable of multi-scale live imaging in one setup.
We demonstrate a passively all-normal dispersion mode-locked Nd-doped fiber laser at 905–940 nm with high optical to optical efficiency. The central wavelengths can be tunable through tuning birefringent filter.
Super-continuum light (1050–1700nm at the 75-dB level) was generated from femtosecond pulse of a mode-locked Er-doped fiber laser system. The system included the oscillator, Er-doped fiber amplifier (EDFA) and the spectral broadening system. A new type of fiber with an amplification of 10dB/km was applied in EDFA. The output pulse energy after amplification was 2.85 nJ with average power of 350 mW...
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