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Age prediction based on methylation analysis has been reported in many populations, with 10 ng or more of DNA usually required for each determination. In this study, we designed thermostable locked nucleic acid (LNA) primers by replacing a small number of DNA bases in standard DNA primers with LNAs. We evaluated these primer sets by single‐base extension analysis using 10, 5, or 2 ng of DNA that would...
This paper proposes direct parameters tuning method which is extension of FRIT (Fictitious Reference Iterative Tuning) for disturbance attenuation on measurable disturbance feed-forward control combined with PID control system and evaluates the variance of system output as an index of disturbance attenuation performance with numerical simulations to illustrate the efficiency of the proposed method.
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