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We proposed on-chip spatiotemporal sensing for monitoring interactions between cells and environment using fluorescence multi-microsensors. The microfluidic chip integrating fluorescence multi-microsensor is used to construct the harmonious environment like in vivo to reconstruct high-quality organs ex vivo. Fluorescence sensing is suitable for spatiotemporal sensing with high spatial resolution because...
Effective manipulation and rapid injection of nanosensor with high cell viability have great significance to biological and biomedical applications such as drug delivery and cells biology. This study presents manipulation of a magnetic nanosensor with a temperature indicator and immobilization onto cell surface using glass nanoprobe by Coulomb's force. The nanosensor is stained by Rhodamine B as the...
Rapid and effective injection of functional nanobeads into a specific living cell with high cell viability is one of the most important issues for single cell analysis. This paper reports a novel method of injecting single magnetic nanobeads into a living Madin-Darby canine kidney (MDCK) cell using a continuous-wave near infrared laser. Experimental results demonstrated the successful injection of...
In this paper, we propose the selective adhesion and rapid injection of a fluorescent sensor into a target cell via the optical control of zeta potential and local vibration stimulus using optical tweezers. A multi-fluorescent sensor, which can respond to both temperature and pH, was encapsulated in anionic lipid layers containing a photochromic material (spiropyran) via the layer-by-layer method...
Evaluation of oocyte and embryo has become important for many biomedicai applications. We developed measurement of oxygen consumption rate of individual oocyte using fluorescence oxygen sensor having stripe-shape on a microfluidic chip. The fluorescence sensor was made of the mixture of polyethylene glycol diacrylate and Tris (2,2'-bipyridyl) dichloro ruthenium (II) hexahydrate (Ru(bpy)3Cl2). The...
We synthesized a novel multi-fluorescence sensor which can respond to both pH and temperature. This kind of sensor was based on amino-polystyrene microbeads and two different fluorescent indicators (Rhodamine B and FITC) were applied simultaneously to synthesize the multi-sensor. Rhodamine B shows an excellent linear relationship between the relative fluorescent intensity and temperature while it...
We developed selective transfection of single fluorescence sensor into a specific cell by optically controlled cell immobilization and membrane fusion of lipid capsule for intracellular measurement. The single fluorescent sensor was encapsulated in the lipid capsule using novel packaging based on a multi-layering method. The liposomes with number-controlled lipid bulyer contain 1,3,3-Trimethylindolino-6'-nitrobenzopyrylospiran...
In this paper, we developed transfection of a single fluorescent nanosensor into a cell nucleus using step-wise fusion of liposome for cellular temperature measurement. The single fluorescent temperature nanosensor is included in the multi lamellar liposomes using Layer-by-Layer formation. The sensor is manipulated by optical tweezers and transfected into the cell or cell nucleus by membrane fusion...
We developed a novel temperature measurement and control method using temperature dependence of quantum dots (Q-dots). We used hydrogel-tool containing Q-dots for temperature measurement and control. These tools can be manipulated in a solution by optical tweezers. We used image processing to obtain color information for measuring the temperature. We calibrated the temperature using the gel-tool in...
In this paper, we proposed cell investigation tool for measurement of physiological conditions of single cell. We developed a novel temperature measurement method using temperature dependence of the quantum dot. The cell investigation tool encapsulates the quantum dot and adheres to the cell membrane. These tools can be manipulated in a solution by optical tweezers. We proposed image processing of...
In this paper, we propose new evaluation method for thermal conductivity measurement of single carbon nanotube (CNT) in liquid with hydrogel sensor containing quantum dot (Q-dot). Measurement of thermal property of single CNT in liquid is quite difficult because escape of the heat from measurement device. Therefore, novel evaluation method using the hydrogel sensors for both thermal input and temperature...
In this paper, we proposed a long-lifetime temperature measurement using color shift of the fluorescence from the quantum dots. The cell investigation tool encapsulates the quantum dots and can adhere to the cell membrane. These tools can be manipulated in a solution by optical tweezers. We used image processing of fluorescent image of the tools to acquire the color information from the fluorescent...
We developed Optical pH regulation using gel tool impregnated with photochromic material for selective cell injection of nanosensor for intracellular measurement. The nanosensor was included in the fusogenic liposome. Membrane fusion of the liposome to the cell membrane was used for invasive cell injection of the nanosensor. The liposome fuses to the cell in weak acidic condition. Local pH regulation...
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