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Deer blood albumin was first separated with membrane technology. Microfiltration as pretreatment methods greatly improved the separation efficiency of ultrafiltration. The major factors of ultrafiltration such as pressure, temperature, PH and material-liquid total protein were investigated to evaluate the effect on membrane flux. The optimal operating condition was 0.2 MPa of operating pressure, 35°C...
An enzyme-linked immunosorbent assay (ELISA) was used to detect the content of insulin-like growth factor-1 (IGF-1) in velvet antler water extract. The ELISA was validated with respect to parallelism, recovery, intra-assay variation, and inter-assay variation. The practical working range of detection was estimated to be 0.8 to 200 ng/mL, and the recovery was 96.7% or more. The effect of sample matrix...
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