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In medical image processing, the segmentation of overlapping nuclei is one of the challenging topics, which relates to its application in diagnostic pathology. To achieve the quantification accuracy (ACC) of the diagnosis, we propose an overlapping nuclei segmentation algorithm using the principle of direction‐based flow tracking (DBFT). The DBFT, which consists of direction field preparation and...
This paper presents a novel algorithm based on the gradient direction to separate the multiple attaching and overlapping objects. The idea is based on the fact that the gradient directions of north,east,south,and west neighbourhood pixels are divergent when the gradient direction of the pixel located in the boundary region is used as a reference line.The proposed algorithm is composed of three steps:image...
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