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An integrated microcell culture system to control physical conditions in a microenvironment is proposed. The authors quantitatively evaluated the cytotoxicity of commonly used MEMS materials, that is, glass, negative photoresist SU-8, polystyrene and poly(dimethylsiloxane), by using the proposed system. In consequence, in these materials were not seen the cytotoxicity regarding the proliferation....
In contrast to optical tweezers methods, we established a method to evaluate the adhesion force between a bead carried by multiple kinesins and a microtubule. The method also enables us to estimate the number of kinesins involved in the bead transport. Torque Γ and drag force FD applied by the shearing flow were derived theoretically by the flow rate necessary to break kinesin-microtubule bindings...
We present a microfluidic device integrated with a Total Internal Reflection (TIR)-based chip for cell observation and analysis, which enables Total Internal Reflection Fluorescence Microscopy (TIRFM). By integrating the microfluidic device with optical components, the assembly becomes unnecessary. An evanescent field generated by the TIR-based chip was demonstrated by observation of fluorescent beads...
In this paper, we present imaging of the physiological cell reaction of stimulus-responsive cells using a new orifice channel chip named the TISSUE-MIMICKING IN VITRO ANALYSIS SYSTEM. The chip enables designated cells treatment using orifice-channels similar to blood vessels, and cell-array like a tissue: cells are along on the blood vessels. These cell handling is an important part in the observation...
In this paper, we developed a novel device for the analysis of intracellular response to external stimulus in single-cell level with high-spatial resolution that simulates in vivo tissue micro-environment. We utilized multidirectional UV lithography technique to realize simple 3-dimensional fabrication of the tissue-mimicking device. Microchannel with an aperture at its sidewall was fabricated. Localized...
This paper presents a novel technique of single-cell array. For easy handling in biological experiments, we developed open-to-air flow well with pocket-like microstructures. Cells are positioned at the microstructures, one at a place, by the use of electroosmotic flow that provides constant and controllable hydrodynamic drag force for the transport of the cells. Precise positioning of mammalian cells...
In this paper, we propose a multiple cell treatment array with high-density arrayed micro-orifice having an individually accessible microchannel and electrodes. More than 10000 micro-orifices with the diameter of 2 mum can be simultaneously fabricated by Single-Mask inclined UV photolithography for embedded network (SIMPLE) process with the oxygen plasma etching. More than 200 living cells immobilized...
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