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Alors que les techniques d’asservissement respiratoire ont démontré leur bénéfice dosimétrique pour les organes intrathoraciques, leur pertinence selon le lobe pulmonaire atteint n’a pas encore été définie. L’objectif de cette étude était de réaliser une comparaison de volumes et de déplacements des différents lobes pulmonaires entre une acquisition en respiration libre et une acquisition en blocage...
Comprehensive and training simulators that provide realistic visual and haptic feedback during cell indentation tasks are currently investigated. Complex cell geometry inherent to biological cells and intricate mechanical properties drive the need for precise mechanical and numerical modeling to assure accurate cell deformation and force calculations. Advances in alternative finite element formulation,...
In order to understand and characterize the mechanical property and response of the mouse embryonic stem cells (mESC), we used an atomic force microscope (AFM) combined with a PHANToM haptic feedback device. Atomic force microscopy has rapidly become a valuable tool for quantifying the biophysical properties of single cells or a collection of cells through force measurements. We report herein the...
Current cell detection techniques are antibody staining of specific protein markers, morphometric parameters and transgenic markers. These assays are often qualitative and do not quantitatively define the outcome of a cell progression during differentiation. Consequently, we propose to characterize the mechanical behavior of embryonic stem cell, which will predict its stage of differentiation during...
In current AFM-based nanomanipulation systems, the commercial position closed-loop controller for piezoelectric nanopositioning stages are implemented with success in a wide range of industrial applications. Even if these controllers operate with satisfactory nominal tracking performance, considerable attention has been focused on appropriate control strategies to compensate hysteresis, nonlinearities,...
Real time simulation of deformable object is increasingly important to simulate the behavior of the biological cell and a key challenge of deformable simulation is to satisfy the conflicting requirements of real-time interactivity and physical realism. This paper presents methodologies for dynamic modeling, visual/haptic display and model validation of cell injection. We first investigate the challenging...
To facilitate training of biological cell injection operations, we are developing an interactive virtual environment to simulate needle insertion into biological cells. A key challenge of deformable simulation is to satisfy the conflicting requirements of real-time interactivity and physical realism. This paper presents methodologies for dynamic modeling, visual/haptic display and model validation...
This paper presents a new three-dimensional biomicromanipulation system for biological objects such as embryos, cells or oocytes. As the cell is very small, kept in the liquid, and observed through a microscope, the two-dimensional visual feedback makes difficult accurate manipulation in the 3-D world. To improve the manipulation work, we proposed an augmented human-machine interface. A 3-D visual...
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