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Microbubbles were isolated by size using differential centrifugation, and the effect of microbubble size on the acoustics and kinetics of contrast ultrasound imaging was measured in the mouse kidney. Microbubbles in the 1-2 ??m diameter size range comprise over 90% of the microbubbles in freshly generated and commercially available lipid-coated microbubbles. These smaller microbubbles attenuated strongly...
Register file access falls on the critical path of a microprocessor because large heavily ported register files are used to exploit more parallelism. In this paper, we focus on reducing register file complexity by reducing the number of register file read ports. The goal of this paper is to explore the limits of read port reduction in a centralized integer register file i.e. how few read ports can...
Delivery of antisense oligonucleotides, AON, presents many of the same challenges as delivery of any nucleic acid: charge, stability, cell uptake, endolysosomal escape, and entry into the nucleus. Here we demonstrate efficient delivery of AON after loading into biodegradable polymer vesicles or 'polymersomes'. We focus on AON delivery to muscle cells in vitro and in vivo because of the emergence of...
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